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Context-dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila

Genetics. 2023-08; 
Janet Lesley Brown, Joshua D Price, Maksim Erokhin, Judith A Kassis
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Proteins, Expression, Isolation and Analysis … Repair plasmids were 26 synthesized by GenScript and included the WT or mutated 1.5kb region plus 1kb homology arms 27 on either side. Repair plasmids were made for WT and for … Get A Quote

摘要

Polycomb group (PcG) proteins maintain the silenced state of key developmental genes, but how these proteins are recruited to specific regions of the genome is still not completely understood. In Drosophila, PcG proteins are recruited to Polycomb response elements (PREs) comprised of a flexible array of sites for sequence-specific DNA binding proteins, "PcG recruiters," including Pho, Spps, Cg, and GAF. Pho is thought to play a central role in PcG recruitment. Early data showed that mutation of Pho binding sites in PREs in transgenes abrogated the ability of those PREs to repress gene expression. In contrast, genome-wide experiments in pho mutants or by Pho knockdown showed that PcG proteins can bind to PREs in... More

關(guān)鍵詞

Polycomb, Polycomb response element (PRE), chromatin context
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