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The Tip Region on VP2 Protein of Bluetongue Virus Contains Potential IL-4-Inducing Amino Acid Peptide Segments

Pathogens. 2020-12; 
Jia-Ling Yang, Chia-Yi Chang, Chih-Shuan Sheng, Chia-Chi Wang, Fun-In Wang
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Catalog Antibody BTV signals were labeled by rabbit anti-BTV-NS3 antibody (GenScript, Piscataway, NJ, USA) Get A Quote

摘要

Bluetongue is an infectious viral hemorrhagic disease of domestic and wild ruminants that has a considerable economic impact on domestic ruminants. There are currently at least 29 serotypes of bluetongue virus (BTV) in the world. Noteworthily, the pathogenesis among BTV serotypes is different, even in the same animal species. In this study, BTV2/KM/2003 and BTV12/PT/2003 were used to investigate the differential immunological effects on bovine peripheral blood mononuclear cells (PBMCs). The BTV viral load and the expression of cytokine messenger RNA (mRNA) in PBMCs were measured by fluorescence-based real-time reverse-transcription PCR (qRT-PCR). The immunofluorescence assay (IFA) was applied to detect BTV sign... More

關鍵詞

IL-4, bioinformatics, bluetongue virus (BTV), cytokines, monocyte-derived macrophage (MDM), pathogenesis, peripheral blood mononuclear cell (PBMC)
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