| Products/Services Used | Details | Operation |
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| Catalog Antibody | … Finally, a total protein sample of 80 μg/well was analyzed by Western blot. The primary antibodies were anti-Hsp16.3 (Genscript, China) and anti-CD63 (Boster, China) antibody. The results of each experiment were repeated three times. Results?… | Get A Quote |
Exosomes are selectively packaged cell-derived vesicles that contain a rich cargo of nucleic acids and proteins. The small heat shock protein, Hsp16.3, is an important capsule protein produced by Mycobacterium tuberculosis (MTB). Exploring the distribution of Hsp16.3 in exosomes is valuable to tuberculosis biomarker development. Our results showed that Hsp16.3 protein overexpressed in cells can be efficiently packaged into exosomes. U937 cells infected with MTB secreted abnormally excessive amounts of Hsp16.3 protein in exosomes. Finally, a substantial number of Hsp16.3 proteins were detected in blood exosomes of tuberculosis patients. The research provides a potential exosome-based tuberculosis biomarker for M... More
