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Lipopolysaccharide Binding Protein and CD14, Cofactors of Toll-like Receptors, Are Essential for Low-Grade Inflammation-Induced Exacerbation of Cartilage Damage in Mouse Models of Posttraumatic Osteoarthritis

Arthritis Rheumatol. 2021-07; 
Yoonkyung Won, Jeong-In Yang, Seulki Park, Jang-Soo Chun
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Recombinant Proteins … On culture day 2, cells at passage 0 were treated with IL‐1β (GenScript) in complete medium or with IL‐6 (Merck Millipore) and LPS (Sigma‐… Gene ratio is the number of core enriched genes divided by number of genes in a given KEGG gene set after filtering out the genes that … Get A Quote

摘要

objective: Osteoarthritis (OA) is initiated by pathogenic factors produced by multiple stimuli, including mechanical stress, metabolic stress, and/or inflammaging. This study was undertaken to identify novel low-grade inflammation-associated pathogenic mediators of OA. methods: Candidate pathogenic molecules were screened using microarray data obtained from chondrocytes exposed to OA-associated catabolic factors. In mice with OA generated by destabilization of the medial meniscus (DMM), low-grade inflammation was induced by a high-fat diet or endotoxemia. Functions of candidate molecules in OA pathogenesis were examined using primary-culture chondrocytes from mice with DMM-induced OA, following intraarticular i... More

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