Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
| Products/Services Used |
Details |
Operation |
| Recombinant Proteins |
… Surrogate virus microneutralization test (sVNT). sVNT tests (GenScript USA, Inc., NJ, USA)
were performed following the manufacturer's instructions?… Discussion. We compared the
GenScript sVNT assay to cVNT, PRNT and commercial CLIA assays?… |
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We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75?% concordant overall and 80?% concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies' neutralizing capacity outside CL-3 laboratory containment.
We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75?% concordant overall and 80?% concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies' neutralizing capacity outside CL-3 laboratory containment.
COVID-19, PRNT, SARS CoV-2, enzyme immunoassay, microneutralization, neutralization, serology
