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A novel DNA platform designed for vaccine use with high transgene expression and immunogenicity

Vaccine. 2021-11; 
George Giorgi Babuadze, Jose Echanove, Claude Lamarre, Marc-Antoine deLaVega, Hugues Fausther-Bovendo, Trina Racine, Alejandro M Gomez, Hiva Azizi, Mathew Wade, Robert Kozak, Gary P Kobinger
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Catalog Antibody … This sequence was submitted to GenScript for synthesis. Next, the orientation of the ORI-Neo/Kan cassette was reversed and obtained backbone was named as pIDV. In order to make a better version of pIDV backbone additional changes were made, specifically nucleotides … Get A Quote

摘要

The development of new, low-cost vaccines and effective gene therapies requires accurate delivery and high-level expression of candidate genes. We developed a plasmid vector, pIDV-II, that allows for both easy manipulation and high expression of exogenous genes in mammalian cells. This plasmid is based upon the pVax1 plasmid and shares a common structure with typical mammalian transcription units. It is composed of a chicken β-actin promoter (CAG), followed by an intron and flanked by two restriction sites, and also includes a post-transcriptional regulatory element, followed by a transcriptional termination signal. While the modification of pVax1 elements either decreased eGFP expression levels or had no effe... More

關鍵詞

DNA, Immunogenicity, Novel plasmid, Vaccine, Viral antigens
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