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Application of Human Induced Pluripotent Stem Cell-Derived Intestinal Organoids as a Model of Epithelial Damage and Fibrosis in Inflammatory Bowel Disease

Biol Pharm Bull. 2020; 
Daichi Onozato, Takumi Akagawa, Yuriko Kida, Isamu Ogawa, Tadahiro Hashita, Takahiro Iwao, Tamihide Matsunaga
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Recombinant Proteins … supplement, and SlowFade Diamond antifade mountant, (Thermo Fischer Scientific Inc., Waltham, MA, USA); fetal bovine serum (FBS), Nichirei (Biosciences Inc., Tokyo, Japan); FGF 2, R-spondin 1, noggin, and epidermal growth factor (EGF), (GenScript, Piscataway, NJ, USA … Get A Quote

摘要

Inflammatory bowel disease, which typically manifests as Crohn's disease and ulcerative colitis, is caused by the abnormal production of cytokines such as tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β. These cytokines damage intestinal epithelial cells and trigger fibrosis, respectively, for which the current in vitro models have many limitations. Therefore, we tested whether human induced pluripotent stem cell-derived intestinal organoids (HiOs) can mimic inflammatory bowel disease (IBD), and whether such a model is suitable for drug screening. HiOs were treated with TNF-α and TGF-β to construct mucosal damage and fibrosis models. TNF-α diminished the mRNA expression of intestinal e... More

關(guān)鍵詞

induced pluripotent stem cell, inflammatory bowel disease, intestinal organoid, transforming growth factor (TGF)-β, tumor necrosis factor (TNF)-α
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