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Lyophilized, antigen-bound liposomes with reduced MPLA and enhanced thermostability

Int J Pharm. 2020; 
Moustafa T Mabrouk, Wei-Chiao Huang, Bingbing Deng, Nasi Li-Purcell, Amal Seffouh, Joaquin Ortega, Gunes Ekin Atilla-Gokcumen, Carole A Long, Kazutoyo Miura, Jonathan F Lovell
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Proteins, Expression, Isolation and Analysis … BSA) in PBS containing 0.1% Tween20 (PBS-T) for another 2 h. Later on, mice sera were serially diluted with 1% BSA in PBS-T for 1 h at 37 °C, followed by incubation with horse radish peroxidase-conjugated goat anti-mouse secondary antibody IgG (Genscript # A00160) for?… Get A Quote

摘要

Thermostability and decreased component costs are desirable features for adjuvanted, recombinant vaccines. We previously showed that a model malaria transmission-blocking vaccine candidate antigen, Pfs25, can be rendered more immunogenic when mixed with liposomes containing cobalt porphyrin-phospholipid (CoPoP) and a synthetic monophosphoryl lipid A (MPLA) variant. CoPoP can induce stable particle formation of recombinant antigens based on interaction with their polyhistidine tag. In the present work, different synthetic MPLA variants and concentrations were assessed in CoPoP liposomes. Long-term biophysical stability and immunogenicity were not adversely impacted by a 60% reduction in MPLA content. When admixe... More

關鍵詞

Adjuvants, Liposomes, Lyophilization, Malaria, Thermostability, Vaccines
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