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Vertical shifts of particle-attached and free-living prokaryotes in the water column above the cold seeps of the South China Sea

Mar Pollut Bull. 2020-05-01; 
Yue Zhang, Hongmei Jing, Xiaotong Peng
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Codon Optimization … To construct plasmid pBSA, the codon-optimized genes mvaS and mvaA from Lactobacillus casei were synthesized by GenScript (Nanjing, China). Then, atoB from E. coli and mvaS and mvaA from L. casei were amplified using primer pairs atoB-F/atoB-R, mvaS-F/mvaS-R, and … Get A Quote

摘要

Marine particle-attached (PA) and free-living (FL) microbes play important roles in the biogeochemical cycling of organic matter along the water column. Deep-sea cold seeps are highly productive and chemosynthetic ecosystems, their continuous emission of CH, CO, and HS can reach up to 100?m in the above water, therefore would influence the distribution and potential metabolic functions of deep-sea prokaryotes. In this study, the vertical distribution profiles of both PA and FL microbes in the water column above two cold seeps of the South China Sea were investigated using Illumina sequencing and quantitative PCR (qPCR) based on 16S rRNA gene. Photosynthetic and heterotrophic prokaryotes were predominant in res... More

關鍵詞

Cold seeps, Free-living, Particle-attached, Water column
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