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Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-β1-NOX4 Pathway

J Mol Neurosci. 2020-06-01; 
Xianjun Tan, Tao Li, Shaowei Zhu, Weiying Zhong, Feng Li, Yunyan Wang
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Recombinant Proteins … 1999) under pathological conditions. Thus, 6 μg/ml SPARC (Genscript, Jiangsu, China) was used to simulate overexpression in cells, and the effect at different time periods (0, 3 h, 6 h, 12 h, 18 h, and 24 h) was detected. When … Get A Quote

摘要

Secreted protein acidic and rich in cysteine (SPARC) has a close association with inflammatory response and oxidative stress in tissues and is widely expressed in intracranial aneurysms (IAs), especially in smooth muscle cells. Therefore, it is inferred that SPARC might be involved in the formation and development of IAs through the inflammatory response pathway or oxidative stress pathway. The aim of this study is to investigate the pathological mechanism of SPARC in oxidative stress, inflammation, and apoptosis during the formation of IAs, as well as the involvement of TGF-β1 and NOX4 molecules. Human brain vascular smooth muscle cells (HBVSMCs) were selected as experimental objects. After the cells were sti... More

關(guān)鍵詞

Human brain smooth muscle cells, Intracranial aneurysms, Oxidative stress, Phenotype transformation, SPARC, TGF-β1-NOX4 pathway
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