| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | … GenScript Taq DNA Polymerase (Piscataway, NJ) was used for PCR amplification using two primer sets designed to amplify a 567 nt frag- ment of RNA3 (forward: 5′-ATC CAG TAG TGA ACTCC-3′ and reverse: 5′-CAC CAT CAG GAA CTT GTA ATG TTT -3′) and 377 nt … | Get A Quote |
Raspberry leaf blotch virus (RLBV) is the putative agent of the homonymous disease and even though Bosnia and Herzegovina is a major producer worldwide there is no report of the virus presence in the country. We studied the virus population structure and assessed its ability to move systemically. RLBV is widespread in production areas and has a homogeneous population structure; leading to the hypothesis that the primary mode of dissemination is propagation material. The ability of the virus to move systemically eliminates propagation of root cuttings as a viable option to obtain RLBV-free plants, leaving RT-PCR screening as the better option to propagate RLBV- free plants in the absence of clean-up facilities o... More
