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Biomimetic polymeric nanoparticle-based photodynamic immunotherapy and protection against tumor rechallenge.

Biomater Sci. 2020; 
Kim D, Byun J, Park J, Lee Y, Shim G, Oh YK.
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Recombinant Proteins … unit/mL)/streptomycin (100 μg/mL), 20 ng/mL of recombinant mouse granulocyte-macrophage colony stimulating factor (GM-CSF), 20 ng/mL of recombinant mouse interleukin-4 (IL-4; GenScript Biotech Corp, Piscataway, NJ, USA) and 50 μM β- mercaptoethanol (Sigma-Aldrich) … Get A Quote

摘要

In this study, we sought to design a bionanomaterial that could exert anticancer effects against primary tumors and protect against rechallenged tumors via photodynamic immunotherapy. As a biomaterial, we used an amphiphilic phenylalanine derivative of poly-gamma glutamic acid, which forms nanoparticles by self-assembly. For anticancer effects, we co-entrapped hydrophobic chlorin e6 and monophosphoryl lipid A in the core of the plain amphiphilic phenylalanine nanoparticles (AN), to generate M/C/AN. For comparison, we used plain AN and chlorin e6-loaded AN (C/AN). In vitro studies showed that B16F10 cancer cells treated with C/AN or M/C/AN generated reactive oxygen species and exhibited an enhanced surface displ... More

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