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Development of Versatile Vectors for Heterologous Expression in Bacillus.

Microorganisms. 2018; 
Larsen ?, Bjerga GEK.
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Codon Optimization … and verified by sequencing. Codon-optimized apr genes (Genscript, Piscataway, NJ, USA) encoding subtilisins from B. licheniformis DSM13, B. paralicheniformis ATCC 9945A, B. subtilis subsp. subtilis str. 168 and B. amyloliquefaciens … Get A Quote

摘要

The discovery of new enzymes for industrial application relies on a robust discovery pipeline. Such a pipeline should facilitate efficient molecular cloning, recombinant expression and functional screening procedures. Previously, we have developed a vector set for heterologous expression in Escherichia coli. Here, we supplement the catalogue with vectors for expression in Bacillus. The vectors are made compatible with a versatile cloning procedure based on type IIS restriction enzymes and T4 DNA ligase, and encompass an effective counter-selection procedure and complement the set of vectors with options for secreted expression. We validate the system with expression of recombinant subtilisins, which are general... More

關鍵詞

Bacillus; ccdB; cloning; recombinant DNA technology; subtilisin
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