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UPF1/SMG7-dependent microRNA-mediated gene regulation.

Nat Commun. 2019;

Park J, Seo JW, Ahn N, Park S, Hwang J,, Nam JW,.

Products/Services Used	Details	Operation
Catalog Antibody	The speci?c proteins were probed with antibodies against the following proteins: FLAG (GenScript, Piscataway, NJ, USA, A00187-100, 1:1000), UPF1 (Cell Signaling Tech- nology, Danvers, MA, USA, #9435, 1:1000), Dicer (Cell Signaling Technology, #5362, 1:1000), SMG7 (Bethyl Laboratories, Montgomery, TX, USA, A302-170A, 1:1000), β- actin (Sigma, A2228, 1:2000), PABP (Santa Cruz Biotechnology Dallas, TX, USA, sc- 28834, 1:500), Ago2 (Abnova, Taipei, Taiwan, H00027161-M01, 1:1000), NOT1 (Proteintech, Rosemont, IL, USA, 14276-1-AP, 1:500), NOT3 (Abcam, Cambridge, Cambridgeshire, UK, ab154276, 1:500), TNRC6A (Abcam, Cambridge, Cambridge- shire, UK, ab156173, 1:500) TNRC6C (Bethyl Laboratories, Montgomery, TX, USA, A303-969A, 1:500) and Calnexin (Santa Cruz Biotechnology, Dallas, TX, USA, sc- 11397, 1:1000).	Get A Quote

摘要

The stability and quality of metazoan mRNAs are under microRNA (miRNA)-mediated and nonsense-mediated control. Although UPF1, a core mediator of nonsense-mediated mRNA decay (NMD), mediates the decay of target mRNA in a 3'UTR-length-dependent manner, the detailed mechanism remains unclear. Here, we suggest that 3'UTR-length-dependent mRNA decay is not mediated by nonsense mRNAs but rather by miRNAs that downregulate target mRNAs via Ago-associated UPF1/SMG7. Global analyses of mRNAs in response to UPF1 RNA interference in miRNA-deficient cells reveal that 3'UTR-length-dependent mRNA decay by UPF1 requires canonical miRNA targeting. The destabilization of miRNA targets is accomplished by the combination of Ago2 ... More