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O-GlcNAcylation is required for B cell homeostasis and antibody responses.

Nat Commun. 2017;

Wu JL, Chiang MF, Hsu PH, Tsai DY,, Hung KH, Wang YH,, Angata T, Lin KI.

Products/Services Used	Details	Operation
Catalog Antibody	1038/s41467-017-01677-z AR T IC L E 1:1000), anti-PI3K p85 (Cell Signaling, #4228, 1:1000), anti-phospho-AKT (Ser473) (Cell Signaling, #9271, 1:1000), anti-AKT (Cell Signaling, #9272, 1:1000), anti- phospho-CD79a (Tyr182) (Cell Signaling, #5173, 1:1000), anti-CD79a (Abcam, ab3121, 1:1000), anti-Bim (Millipore, AB1770S, 1:1000), anti-Erk5 (Cell Signaling, #3372, 1:1000), anti-calnexin (Santa-Cruz, sc-6465, 1:1000), anti-OGT (Genetex, GTX30868, 1:1000), anti-actin (Sigma, A2228, 1:5000), anti-tubulin (Thermo, MS- 581, 1:1000), anti-Flag (Sigma, F3165, 1:1000), and anti-myc (GenScript, A00172, 1:1000).	Get A Quote

摘要

O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) catalyzes O-GlcNAc modification. O-GlcNAcylation is increased after cross-linking of the B-cell receptor (BCR), but the physiological function of this reaction is unknown. Here we show that lack of Ogt in B-cell development not only causes severe defects in the activation of BCR signaling, but also perturbs B-cell homeostasis by enhancing apoptosis of mature B cells, partly as a result of impaired response to B-cell activating factor. O-GlcNAcylation of Lyn at serine 19 is crucial for efficient Lyn activation and Syk interaction in BCR-mediated B-cell activation and expansion. Ogt deficiency in germinal center (GC) B cells also results in enhanced apopto... More