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Functional Analysis of Kinases and Transcription Factors in Saccharomyces cerevisiae Using an Integrated Overexpression Library.

G3 (Bethesda). 2017; 
Youn JY,, Friesen H, Nguyen Ba AN, Liang W, Messier V, Cox MJ,, Moses AM,, Andrews B,.
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Recombinant Proteins 2) in YPD, then arrested in G1 phase with 5 mM a-factor (GenScript) for 2. Get A Quote

摘要

Kinases and transcription factors (TFs) are key modulators of important signaling pathways and their activities underlie the proper function of many basic cellular processes such as cell division, differentiation, and development. Changes in kinase and TF dosage are often associated with disease, yet a systematic assessment of the cellular phenotypes caused by the combined perturbation of kinases and TFs has not been undertaken. We used a reverse-genetics approach to study the phenotypic consequences of kinase and TF overexpression (OE) in the budding yeast, Saccharomyces cerevisiae We constructed a collection of strains expressing stably integrated inducible alleles of kinases and TFs and used a variety of ass... More

關(guān)鍵詞

genetic interactions; genetic networks; kinase; transcription factor; yeast genetics
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