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Catch and Release of Cytokines Mediated by Tumor Phosphatidylserine Converts Transient Exposure into Long-Lived Inflammation.

Mol Cell. 2017;

Oyler-Yaniv J, Oyler-Yaniv A, Shakiba M, Min NK, Chen YH, Cheng SY, Krichevsky O, Altan-Bonnet N, Altan-Bonnet G.

Products/Services Used	Details	Operation
Recombinant Proteins	e1–e7, June 1, 2017 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chondroitin ABC lyase R&D Systems Cat: 6877-GH-020, CAS: 9024-13-9 Pronase Sigma Aldrich Cat: 53702, CAS: 9036-06-0 JAK1/2 inhibitor AZD1480 Laboratory of Jackie Bromberg (MSKCC) N/A Cyclosporin A Sigma Aldrich Cat: 30024, CAS: 59865-13-3 Dasatinib SRC kinase inhibitor Selleckchem Cat: S1021, CAS: 302962-49-8 MEK kinase inhibitor (PD0325901) Selleckchem Cat: S1036, CAS: 391210-10-9 Cell Trace Violet ThermoFisher Cat: C34557 Cell Trace Far Red ThermoFisher Cat: C34572 CFSE ThermoFisher Cat: C34554 Cell Mask Orange Invitrogen Cat: C10045 Methyl b cyclo dextrin Sigma Aldrich Cat: C332615, CAS: 128446-36-6 Saponin Sigma Aldrich Cat: 47036, CAS:8047-15-2 Filipin III Cayman Chemical Cat: 70440, CAS: 480-49-9 Lovastatin Alfa Aesar Cat: H52792, CAS: 75330-75-5 Fluorescent-labeled Annexin V ThermoFisher Cat: A13201, A13202 Unlabeled Annexin V eBioscience Cat: BMS306 BODIPY-cholesterol Laboratory of Nihal Altan-Bonnet (NHLBI, NIH) N/A Recombinant mouse MFG-E8 R&D Systems Cat: 2805-MF-050 Recombinant mouse TNF-a R&D Systems Cat: 410-MT-050 Streptavidin-Alexa488 Jackson ImmunoResearch Cat: 016-540-084 DAPI Sigma Aldrich Cat: D9542, CAS: 28718-90-3 Fatty acid free bovine serum albumin Sigma Aldrich Cat: 126609, CAS: 9048-46-8 Recombinant mouse IFNg GenScript Cat: Z02916 Carrier-free recombinant mouse IFNg BioLegend Cat: 575306 Recombinant mouse M-CSF GenScript Cat: Z03275 Critical Commercial Assays Cytokine Bead Array Bead-based ELISA BD Biosciences Cat: 551287 RNeasy Mini Kit QIAGEN Cat: 74106 QScript cDNA Supermix Quantas biosciences Cat: 95048 Maxima First-Strand cDNA Synthesis Kit ThermoFisher Cat: K1671 Fast SyBR Green Master Mix ThermoFisher Cat: 4385610 iTaq Universal SyBR Green Supermix Bio-Rad Cat: 172520 Mouse IFNg cytokine secretion assay Miltenyi biotec Cat: 130-090-517 Lipid Strips Echelon Biosciences Cat: P-6002 Microscale Protein Labeling Kit ThermoFisher Cat: A30006, A30009 ECL Western Blotting Substrate Pierce Cat: 32106 Deposited Data RNA sequencing data This Paper GEO: GSE85535 Raw data [Cell imaging + western blotting] This Paper Mendeley Data: https://data.	Get A Quote

摘要

Immune cells constantly survey the host for pathogens or tumors and secrete cytokines to alert surrounding cells of these threats. In?vivo, activated immune cells secrete cytokines for several hours, yet an acute immune reaction occurs over days. Given these divergent timescales, we addressed how cytokine-responsive cells translate brief cytokine exposure into phenotypic changes that persist over long timescales. We studied melanoma cell responses to transient exposure to the cytokine interferon γ (IFNγ) by combining a systems-scale analysis of gene expression dynamics with computational modeling and experiments. We discovered that IFNγ is captured by phosphatidylserine (PS) on the surface of viable cells b... More