| Products/Services Used | Details | Operation |
|---|---|---|
| Monoclonal Antibody Services | 8 Developmental Cell 44, 1–8, February 26, 2018 STAR+METHODS KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-beta-galactosidase Developmental Studies Hybridoma Bank Cat# 40-1a; RRID: AB_528100 Mouse monoclonal anti-Singed (fascin) Developmental Studies Hybridoma Bank Cat# sn 7C; RRID: AB_528239 Mouse monoclonal anti- Fasciclin III Developmental Studies Hybridoma Bank Cat# 7G10; RRID: AB_528238 Mouse monoclonal anti-alpha-tubulin Developmental Studies Hybridoma Bank Cat# 12G10; RRID: AB_1157911 Mouse monoclonal anti-HA-probe Santa Cruz Biotechnology Cat# sc-7392; RRID: AB_627809 Mouse monoclonal anti-c-Myc Santa Cruz Biotechnology Cat# sc-40; RRID: AB_627268 Rabbit polyclonal anti-GFP (FL) Santa Cruz Biotechnology Cat# sc-8334; RRID: AB_641123 Protein A/G PLUS-Agarose antibody Santa Cruz Biotechnology Cat# sc-2003; RRID: AB_10201400 Mouse monoclonal anti-FLAG M2 Sigma-Aldrich Cat# F1804; RRID: AB_262044 Rabbit polyclonal anti-phospho-Drosophila Akt (Ser505) Cell Signaling Technology Cat# 4054S; RRID: AB_331414 Mouse monoclonal anti-beta-Actin GenScript Cat# A00702; RRID: AB_914102 ChromoTek GFP-Trap agarose beads ChromoTek Cat# gta-100; RRID: N/A Mouse monoclonal anti-Multi Ubiquitin MBL International Cat# D058-3; RRID: AB_592937 Cy3-Af?niPure Goat Anti-Mouse IgG (H+L) Jackson ImmunoResearch Labs Cat# 115-165-166; RRID: AB_2338692 Alexa Fluor 647-Af?niPure Goat Anti-Rat IgG (H+L) Jackson ImmunoResearch Labs Cat# 112-605-167; RRID: AB_2338404 Alexa Fluor 647-Af?niPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Labs Cat# 111-605-144; RRID: AB_2338078 Peroxidase-Af?niPure Goat Anti-Mouse IgG Jackson ImmunoResearch Labs Cat# 115-035-003; RRID: AB_10015289 Peroxidase-Af?niPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Labs Cat# 111-035-003; RRID: AB_2313567 Neutravidin HRP Thermo Fisher Cat# A2664; RRID: N/A Rabbit polyclonal anti-Slbo Gift from Pernille Rorth (Montell et al.... Primary antibodies used for western blot were mouse anti-GFP (1:5000 Santa Cruz), mouse anti-beta-actin (1:10000 Genscript), mouse anti-alpha-tubulin (1:10000 Santa Cruz), mouse anti-HA (1:5000, Santa Cruz) and mouse anti-Myc (1:5000, Santa Cruz), rabbit anti-p-dAkt (1:5000, CST), rabbit anti-t-dAkt (1:5000, from Tien Hsu) and mouse anti-Flag (1:50000, Sigma), and mouse anti- Multi-Ubiquitin (MBL International). | Get A Quote |
Cell growth and cell differentiation are two distinct yet coupled developmental processes, but how they are coordinated is not well understood. During Drosophila oogenesis, we found that the growth-promoting InR/Akt/TOR pathway was involved in suppressing the fate determination of the migratory border cells. The?InR/Akt/TOR pathway signals through TOR and Raptor, components of TORC1, to downregulate the?JAK/STAT pathway, which is necessary and sufficient for border cell fate determination. TORC1 promotes the protein stability of SOCS36E, the conserved negative regulator of JAK/STAT signaling, through physical interaction, suggesting that TORC1 acts as a key regulator coordinating both cell growth and cell dif... More
