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Transposon-mediated generation of CAR-T cells shows efficient anti B-cell leukemia response after ex vivo expansion

Gene Ther. 2020; 
Chicaybam L,, Abdo L, Viegas M, Marques LVC, de Sousa P, Batista-Silva LR, Alves-Monteiro V, Bonecker S, Monte-Mór B, Bonamino MH,.
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Codon Optimization … Hospital, Memphis, TN) This sequence was codon optimized and synthesized by Genscript (Piscataway, NJ) and was cloned in the transposon vector pT3 (kindly provided by Dr Richard Morgan, NIH) using AgeI/NotI sites A myc … Get A Quote

摘要

CAR-T-cell therapy has shown considerable advance in recent years, being approved by regulatory agencies in US, Europe, and Japan for the treatment of refractory patients with CD19+ B-cell leukemia or diffuse large B-cell lymphoma. Current methods for CAR-T-cell production use viral vectors for T-cell genetic modification and can take up to 15 days to generate the infusion product. The development of simple and less costly manufacturing protocols is needed in order to meet the increasing demand for this therapy. In this present work, we generated 19BBz CAR-T cells in 8 days using a protocol based on the non-viral transposon-based vector Sleeping Beauty. The expanded cells display mostly a central memory phenoty... More

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