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Rapid determination of quaternary protein structures in complex biological samples.

Nat Commun. 2019; 
HauriSimon,KhakzadHamed,HapponenLotta,TelemanJohan,Malmstr?mJohan,Malmstr?m
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Recombinant Proteins Page 1. ARTICLE Rapid determination of quaternary protein structures in complex biological samples Simon Hauri 1, Hamed Khakzad 2,3, Lotta Happonen1, Johan Teleman1, Johan Malmstr?m 1 & Lars Malmstr?m 1,2,3 The …). The encoding sequence was ordered as a synthetic construct from Genscript (NJ, USA) Get A Quote

摘要

The understanding of complex biological systems is still hampered by limited knowledge of biologically relevant quaternary protein structures. Here, we demonstrate quaternary structure determination in biological samples using a combination of chemical cross-linking, high-resolution mass spectrometry and high-accuracy protein structure modeling. This approach, termed targeted cross-linking mass spectrometry (TX-MS), relies on computational structural models to score sets of targeted cross-linked peptide signals acquired using a combination of mass spectrometry acquisition techniques. We demonstrate the utility of TX-MS by creating a high-resolution quaternary model of a 1.8 MDa protein complex composed ... More

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