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Construction of Integration Vectors for Secreted Expression of Bacterial Phytase in Yarrowia Lipolytica

Helix. 2017;

Daria S. Troshagina* , Anna E. Smirnova , Catherine Madzak , Aliya D. Suleimanova

Products/Services Used	Details	Operation
Codon Optimization	… (http://www.genscript.com/codon-opt.html). The codon-optimized genes were synthesized and cloned into pUC57 vector by Genscript (USA). In order to estimate the coding potential of considered DNA sequence, the frequency of optimal codons (FOP) is used …	Get A Quote

摘要

Yeast Yarrowia lipolytica is a handy tool for efficient production of heterologous proteins. In this work we report the construction of integration vectors for secretion of bacterial phytase by t hese yeasts. Pantoea sp. 3.5.1 histidine acid phytase encoding gene sequence was codon-optimized and chemically synthetized. Optimized and native phytase gene sequences were cloned into integrative vector pINA1296, containing signal sequence of XPR2 gene. Vectors were multiplied in E.coli DH5?, isolated and linearized for successful integration into Y.lipolytica genome by homologeous recombination in pBR-region. Y.lipolytica strains with integrated bacterial native and optimized phytase genes were obtained