HGF, Human

Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.

價(jià)格	￥600
產(chǎn)品編號(hào)	Z03229
規(guī)格	10 μg 50 μg 1 mg 大單詢(xún)價(jià)
數(shù)量

產(chǎn)品簡(jiǎn)介圖例技術(shù)背景

產(chǎn)品簡(jiǎn)介

Species

Human

Protein Construction

HGF (Gln32-Ser728)
Accession # P14210

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 10.0 ng/ml, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1.0 × 10⁵ units/mg.

Expression System

CHO

Apparent Molecular Weight

88~90 kDa (single chain), 59~61 kDa (alpha chain), 30~34 kDa (beta chain), on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

ED₅₀ < 10.0 ng/ml, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1.0 × 10⁵ units/mg. ?

Lane 1: 2 μg of HGF, Human, non-reducing (NR)
> 95% as analyzed by SDS-PAGE ?

技術(shù)背景

Target Background

Synonyms

HGF; DFNB39; F-TCF; HGFB; HPTA; SF; hepatocyte growth factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相關(guān)文獻(xiàn)

1. SuZhipeng, et al. Anti-MET VHH Pool Overcomes MET-Targeted Cancer Therapeutic Resistance. Mol. Cancer Ther. (2019)

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HGF, Human

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