SCF, Mouse

Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. It stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. SCF plays an important role in the hematopoiesis during embryonic development. SCF can regulates HSCs in the stem cell niche in the bone marrow. SCF has been shown to increase the survival of HSCs in vitro and contributes to the self-renewal and maintenance of HSCs in-vivo.

價格	￥600
產品編號	Z02997
規格	10 μg 50 μg 1 mg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Mouse

Protein Construction

Expressed with an N-terminal Met.

SCF (Lys26-Ala189)
Accession # P20826

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 10.0 ng/ml, measured by a cell proliferation assay using human TF-1 cells, corresponding to a specific activity of > 1.0 × 10⁵ units/mg.

Expression System

P. pastoris

Apparent Molecular Weight

~18.4 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

ED??< 10.0 ng/ml, measured by a cell proliferation assay using human TF-1 cells, corresponding to a specific activity of > 1.0× 10?units/mg. ?

技術背景

Target Background

Synonyms

Kit ligand; C-Kit Ligand; Mast Cell Growth Factor; MGF; Steel Factor; Stem cell factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相關文獻

1. LawagAbdalla A, et al. HSP90 Inhibition and Cellular Stress Elicits Phenotypic Plasticity in Hematopoietic Differentiation. Cell Reprogram. (2017-10)

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SCF, Mouse

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