G-CSF, Human(CHO-expressed)

Human Granulocyte Colony Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells, the secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.

價格	￥600
產品編號	Z02980
規格	10 μg 50 μg 1 mg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Human

Protein Construction

G-CSF (Thr27-Pro200)
Accession # Q8N4W3

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.1 ng/ml, determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells, corresponding to a specific activity of > 1.0 × 10⁷ units/mg.

Expression System

CHO

Apparent Molecular Weight

~18.7 kDa, on SDS-PAGE under non-reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

ED??< 0.1 ng/ml, determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells, corresponding to a specific activity of >1 x 10? units/mg. ?

技術背景

Target Background

Synonyms

CSF3; C17orf33; CSF3OS; GCSF; colony stimulating factor 3; CSF-3; Granulocyte Colony-Stimulating Factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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G-CSF, Human(CHO-expressed)

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