VEGF165, Human

Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.

價格	￥600
產品編號	Z02689
規格	10 μg 50 μg 100 μg 1 mg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Human

Protein Construction

VEGF165 (Ala27-Arg191)
Accession # P15692-4

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.5 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ of 1.0-5.0 ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2.0 × 10⁵-1.0 × 10⁶ units/mg.

Expression System

P. pastoris

Apparent Molecular Weight

~ 38-40 kDa, on SDS-PAGE under non-reducing conditions
~ 18-20 kDa, on SDS-PAGE under reducing conditions
Structure/form: Disulfide-linked homodimer

Formulation

Lyophilized after extensive dialysis against 25 mM HEPES, 150 mM NaCl, pH 7.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

GenScript product showed better activity compared to competitor P ?

GenScript product showed better activity compared to competitor S ?

GenScript product showed better activity compared to competitor R ?

Recombinant VEGF165, Human (Z02689) was resolved by SDS-PAGE stained with coomassie blue, under reducing (R) and non-reducing (N) conditions, showing major bands at ~ 18-20 kDa and ~ 38-40 kDa, respectively. Multiple bands or smear in the gel are caused by glycosylation.

Lane 1: 2 µg of Z02689, reducing (R)
Lane 2: 2 µg of Z02689, non-reducing (N)
> 95% as analyzed by SDS-PAGE ?

Biological Activity
VEGF-A165, Human (Cat. No. Z02689) stimulates cell proliferation of HUVEC cells. The ED₅₀ for this effect is typically 1-5 ng/mL. ?

技術背景

Target Background

Synonyms

VEGF-165; Vascular Endothelial Growth Factor 165; VPF; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen; Vascular Permeability Factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相關文獻

1. Zhenfeng Chen, et al. Endothelial β-catenin upregulation and Y142 phosphorylation drive diabetic angiogenesis via upregulating KDR/HDAC9. Cell Commun Signal. (2024-03)
2. Nadia O Abutaleb, et al. Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress. STAR Protoc. (2021-03)
3. Graham Rykiel, et al. Transient increase in VEGF-A leads to cardiac tube anomalies and increased risk of congenital heart malformations. Anat Rec (Hoboken). (2021-02)
4. Xu Zhang, et al. In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiological System. Methods Mol Biol. (1905-07)

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VEGF165, Human

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