Measured by its binding ability in a ligand-receptor binding ELISA. When recombinant human 4-1BB Fc Chimera is Immobilized at 1 μg/mL (100 μL/well), the concentration of recombinant human 4-1BB Ligand that produces 50% optimal binding response is found to be approximately 5-15 ng/mL.

4-1BB (CD137) Fc Chimera, Human

4-1BB(CD137) is a member of the tumor necrosis factor (TNF) receptor family. Mature human 4-1BB consists of a 163 amino acid extracellular domain (ECD) with four TNFR cysteine?rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain; 4-1BB (CD137) is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction co stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell. It can also enhance the activation-induced cell death of repetitively stimulated T cells.

產(chǎn)品編號(hào)	Z03382
規(guī)格	50 μg 1 mg 大單詢價(jià)
數(shù)量
價(jià)格	￥1200


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產(chǎn)品簡(jiǎn)介

Species

Human

Protein Construction

4-1BB (Leu24-Gln186) Accession # Q07011	hFc
N-term	C-term

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

Measured by its binding ability in a ligand-receptor binding ELISA. When recombinant 4-1BB/CD137/TNFRSF9, hFc, Human is Immobilized at 1.0 μg/ml (100 μl/well), the concentration of recombinant human 4-1BB Ligand that produces 50% optimal binding response is found to be approximately 5.0-15.0 ng/ml.

Expression System

CHO

Apparent Molecular Weight

55~60 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

技術(shù)背景

Target Background

4-1BB(CD137) is a member of the tumor necrosis factor (TNF) receptor family. Mature human 4-1BB consists of a 163 amino acid extracellular domain (ECD) with four TNFR cysteine?rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain; 4-1BB (CD137) is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4⁺, CD8⁺, memory CD8⁺, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction co stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell. It can also enhance the activation-induced cell death of repetitively stimulated T cells.

Synonyms

4-1BB; TNFRSF9; CD137 antigen; T-cell antigen ILA

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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4-1BB (CD137) Fc Chimera, Human

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