GenScript product showed better activity compared to competitor P

GenScript product showed better activity compared to competitor R

Biological Activity
TNF-alpha, Human (Cat. No. Z01001) stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED₅₀ for this effect is typically 11-50 pg/mL.

2 μg of TNF-α, Human (Cat. No. Z01001) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

TNF-α, Human

Tumor Necrosis Factor-alpha (TNF-a) is a homotrimer with a subunit molecular mass of 17.3 kDa. Tumor Necrosis Factor-alpha(TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.

產(chǎn)品編號	Z01001
規(guī)格	10 μg 50 μg 1 mg 大單詢價
數(shù)量
價格	￥400


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產(chǎn)品簡介

Species

Human

Protein Construction

TNF-α (Val77-Leu233)
Accession # P01375

Purity

> 98% as analyzed by SDS-PAGE
> 98% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 30.0 pg/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 3.3 × 10⁷ units/mg.

Expression System

E. coli

Apparent Molecular Weight

~17.3 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

GenScript product showed better activity compared to competitor P

GenScript product showed better activity compared to competitor R

Biological Activity
TNF-alpha, Human (Cat. No. Z01001) stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED₅₀ for this effect is typically 11-50 pg/mL.

2 μg of TNF-α, Human (Cat. No. Z01001) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

技術(shù)背景

Target Background

Synonyms

TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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TNF-α, Human

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