Multiple Tag (Purified)

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Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. The fusion protein has an apparent molecular weight of ~40 kDa on SDS-PAGE and it contains 16 commonly used tags from N terminus to C terminus in the order as shown in the table below. TagSequenceTrxMSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAT7CMASMTGGQQMGHSVQPELAPEDPEDCc-MycEQKLISEEDLCVSV-gYTDIEMNRLGKGlu-GluCEEEEYMPMEV5GKPIPNPLLGLDSTE-tagGAPVPYPDPLEPRFlag-tagDYKDDDDKS-tagKETAAAKFERQHMDSHAYPYDVPDYACKT3KPPTPPPEPETE2GVSSTSSDFRDRAU1DTYRYIAU5TDFYLK6x HisHHHHHH

產品編號	M0101
規格	大單詢價

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Reconstitution	Add 200 μl of ddH₂O to make the final concentration of 1 μg/μl in PBS Buffer. 0.04-0.2 μl of Multiple Tag is recommended for each lane of the minigel (for accurate loading, dilute with PBS Buffer).
Concentration	200 μg of purified Multiple Tag (lyophilized)
Storage	Store the protein at -20°C or below. After reconstitution, aliquot and store at -20°C or below.

技術背景

Target Background

Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. The fusion protein has an apparent molecular weight of ~40 kDa on SDS-PAGE and it contains 16 commonly used tags from N terminus to C terminus in the order as shown in the table below.

Tag	Sequence
Trx	MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLA
T7	CMASMTGGQQMG
HSV	QPELAPEDPEDC
c-Myc	EQKLISEEDLC
VSV-g	YTDIEMNRLGK
Glu-Glu	CEEEEYMPME
V5	GKPIPNPLLGLDST
E-tag	GAPVPYPDPLEPR
Flag-tag	DYKDDDDK
S-tag	KETAAAKFERQHMDS
HA	YPYDVPDYAC
KT3	KPPTPPPEPET
E2	GVSSTSSDFRDR
AU1	DTYRYI
AU5	TDFYLK
6x His	HHHHHH

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Multiple Tag (Purified)

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