| Product Description |
Recombinant CHO-K1 cells
stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The
surface expression of ACE2 is validated by FACS analysis. This stable cell line
product is designed for cell-based binding assays that measure binding affinity
and stability of antibody based biologics binding with ACE2.
|
| Applications |
Cell-based binding assay; use as immunogen for
antibody development.
|
| Expressed Gene |
Codon
Optimized from NM_021804.2; no expressed tags
|
| Target Protein |
NP_068576.1
|
| Host Cell |
CHO-K1
|
| Quantity |
2 vials of frozen cells (>1X106 per
vial in 1 ml)
|
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received.
|
| Mycoplasma Status |
Negative.
The mycoplasma test was performed with MycoAlert? PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza).
|
| Culture Properties |
Adherent
|
| Freeze Medium |
95% complete growth medium, 5% DMSO (Cat. No. D2650,
Sigma)
|
| Complete Growth Medium |
F-12K (Cat. # 21127-022, Gibco), 10% FBS (Cat.
#10099-141, Gibco)
|
| Culture Medium |
F-12K, 10% FBS, 250 μg/ml Hygromycin B (Cat. No. 10687010,
Invitrogen)
|
| Gene Synonyms |
Angiotensin-converting enzyme 2; ACEH
|
| Background |
Angiotensin-converting
enzyme 2 (ACE2), discovered as a homologue of ACE, acts as its physiological
counterbalance providing homeostatic regulation of circulating angiotensin II
(Ang II) levels. ACE2 is a zinc metalloenzyme and carboxypeptidase located as
an ectoenzyme on the surface of endothelial and other cells. While its primary
substrate appears to be Ang II, it can hydrolyze a number of other
physiological substrates. Additionally, ACE2 functions in other noncatalytic
cellular roles including the regulation of intestinal neutral amino acid
transport. It also serendipitously acts as the receptor for the severe acute
respiratory syndrome virus. Upregulation of ACE2 expression and function is
increasingly recognized as a potential therapeutic strategy in hypertension and
cardiovascular disease, diabetes, lung injury, and fibrotic disorders. ACE2 is
regulated at multiple levels including transcriptional, posttranscriptional
(miRNA and epigenetic), and posttranslational through its shedding from the
cell surface.
|
Figure 1: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells.
CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).
Figure 2: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells from different passages.
The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 represent cell passage numbers.
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