| Product Description |
GenScript BSA ELISA kit (Cat. No. L00944) is a Sandwich ELISA Kit that is designed
for quantitative measurement of bovine serum albumin (BSA) in testing samples. These
outstanding features make it a great solution for routine BSA impurity
detection, quality control and lot release, as well as purification process
optimization.
Most commercial
serum-free media are formulated with various biological macromolecules that can
replace the function of serum, such as bovine serum albumin (BSA), transferrin,
and insulin. The use of serum-free media can greatly reduce the amount of
potential impurities, but the final product serving therapeutic purpose should
be highly purified and quality controlled. Hence, it is necessary to test for trace
media impurities, and the residual concentration of BSA is usually an important indicator for quality control in
production.
|
| Linearity Range |
3 to 64 ng/mL
|
| Sensitivity |
LoD: 0.5 ng/mL
LoQ: 1 ng/mL
|
| Precision |
Intra-assay: CV ≤ 10%;
Inter-assay: CV ≤ 15%
|
| Assay Temperature |
37°C
|
| Kit Contents |
| Component |
Quantity/Size |
Part No. |
| Capture Plate |
1 plate |
F1-80 |
| Standard Stock |
1 vial (50 μL) |
F1-10 |
| Biotin Anti-BSA Antibody |
1 bottle (12 mL) |
F1-20 |
| Streptavidin-HRP |
1 bottle (12 mL) |
E1-30 |
| Sample Dilution Buffer |
1 bottle (12 mL) |
F1-60 |
| TMB Solution |
1 bottle (12 mL) |
A1-40 |
| 20× Wash Solution (2% Tween-20) |
1 bottle (40 mL) |
F1-70 |
| Stop Solution |
1 bottle (6 mL) |
A1-50 |
| Plate Sealer |
2 pieces |
N/A |
|
| Storage |
The unopened kit is stable for at least 12 months from the date of
manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from
the date of opening at 2°C to 8°C.
|
| Assay Principle |
BSA ELISA kit utilizes two anti-BSA monoclonal antibodies which bind to different epitopes of BSA. When the sample and the anti-BSA detection antibody conjugated with biotin are added to the plate (Capture Plate) coated with the anti-BSA capture antibody, the capture antibody and the detection antibody (Biotin Anti-BSA Antibody) bind to the BSA in the sample in a sandwich format. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the Biotin Anti-BSA Antibody. After washing steps, 3,3',5,5'-Tetramethylbenzidine solution (TMB Solution) is added resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm and 650 nm by a microplate reader. The quantity of BSA in the sample is precisely quantified against a BSA standard curve.
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Figure 1: Schematic diagram of BSA ELISA Kit |
For Research Use Only. Not for Use in Diagnostic
Procedures.
