BLI (Biolayer interferometry) binding affinity measurements of MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit to FMC63 scFv.
FMC63 scFv-his captured on Anti-His (HIS) Probes can bind MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) with a dissociation constant (KD) of 7.81E-10M.

Flow cytometric analysis of MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit specificity for FMC63 scFv.
The specificity of the MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit was evaluated using Raji cells as the experimental system. In the first group, Raji cells were incubated with a 1:50 dilution of MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) followed by staining with a PE conjugated anti-rabbit IgG antibody, showing a negative signal (blue line). In the second group, Raji cells were incubated with FMC63 scFv, followed by staining with the same concentration of MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) and PE conjugated anti-rabbit IgG antibody, resulting in a positive signal (red line) .

MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit binds to FMC63 scFv in ELISA assays.
Recombinant FMC63 scFv was coated at a concentration of 1 μg/mL, followed by the addition of MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) at serially diluted concentrations starting from 1 μg/mL. Detection was performed using Mouse Anti-Rabbit IgG Fc Antibody (14H9H10)[HRP], mAb (GenScript, A01856) diluted at 1:20,000.

The detection of PBMCs with or without FMC63 CAR-T cells using iFluor 647 conjugated MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) demonstrated minimal nonspecific binding to PBMCs and specific recognition of FMC63-expressing CARs.

MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit

This product is specific to FMC63 scFv and CARs derived from FMC63 scFv.

產(chǎn)品編號	A02327
規(guī)格	100 Tests 大單詢價(jià)
數(shù)量
價(jià)格	￥12000


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產(chǎn)品概述

Specificity	This product is specific to FMC63 scFv and CARs derived from FMC63 scFv.
Host Species	Rabbit
Immunogen	FMC63 scFv
Conjugate	Unconjugated

應(yīng)用指導(dǎo)

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	1：1,000-1：10,000
Flow Cytometry	1:50

產(chǎn)品屬性

Form	Liquid
Storage Buffer	Supplied in PBS (pH 7.2), 0.03% ProClin300 and 50% glycerol.
Storage Instructions	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Protein A affinity column
Isotype	Rabbit IgG, κ
Clonality	Monoclonal
Clone ID	24B2
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

圖例

技術(shù)背景

Target Background

The FMC63 scFv (single-chain variable fragment) is a key component in many chimeric antigen receptor (CAR) designs, particularly in CAR-T cell therapies. Derived from a murine monoclonal antibody, FMC63 specifically targets human CD19, a cell surface protein highly expressed on B-cells, including malignant B-cells in B-cell leukemias and lymphomas. This scFv fragment is engineered by fusing the variable regions of the heavy and light chains of the antibody, preserving the antigen-binding capability while significantly reducing its size. This smaller, single-chain format enhances its integration into CAR constructs, enabling the recognition and destruction of CD19-positive cells by CAR-T cells. FMC63-based CARs are widely used in immunotherapy, particularly in FDA-approved treatments for B-cell malignancies, such as Kymriah? and Yescarta?.

Synonyms

FMC63; FMC63 scFv; FMC63-scFv; FMC63-CD19; Anti-CD19 (FMC63) CAR; Anti-CD19 (FMC63)

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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MonoRab? Anti-FMC63 Antibody (24B2), mAb, Rabbit

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