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Toll-like receptor 4 is involved in titanium dioxide nanoparticle incorporation into cells

Science of Advanced Materials. 2014-07; 
Mano, Sharmy Saimon; Kanehira, Koki; Sonezaki, Shuji; Taniguchi, Akiyoshi
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Endotoxin Detection & Removal System … The crystal ratio of anatase and rutile of P25 is 80% and 20%, respectively. The Lipopolysac- charide (LPS) contamination in NPs has been checked using ToxiSensor? Chromogenic LAL Endotoxin Assay Kit (GenScript, NJ, USA), according to the manufacturer's instructions … Get A Quote

摘要

The innate immune system is important for defense against foreign pathogens, such as bacteria and viruses. We hypothesized that the innate immune system is also important for the interaction between cells and nanomaterials, because cells do not have a specific defense system for nanomaterials. In this study, we demonstrated the effect of Toll-Like Receptor 4 (TLR 4), which is an important molecule for the innate immune system, on the uptake of TiO2 nanoparticles (NPs). The results indicated that TLR 4 expression vector-transfected cells increased the uptake of TiO2 NPs and also increased IL-6 mRNA induction by TiO2 NP exposure. Modifying TiO2 NPs with polyethylene glycol (PEG) reduced their uptake and inflammat... More

關鍵詞

GENE EXPRESSION; INNATE IMMUNITY; POLY ETHYLENE GLYCOL; SIDE SCATTER; TITANIUM DIOXIDE
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