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Assembling the Tat protein translocase.

Elife. 2016; 
AlcockFelicity,StansfeldPhillip J,BasitHajra,HabersetzerJohann,BakerMatthew Ab,PalmerTracy,WallaceMark I,BerksB
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Polyclonal Antibody Services Polyclonal antibodies against TatA, TatB, and TatC were raised in rabbits by Davids Biotechnologie, Regensburg, Germany (TatA, TatB) or Genscript, Nanjing, China (TatC). Get A Quote

摘要

The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, molecular simulations, and experimentation to define the interactions between the Tat proteins of at molecular-level resolution. In the TatBC receptor complex the transmembrane helix of each TatB molecule is sandwiched between two TatC molecules, with one of the inter-subunit interfaces incorporating a functionally important cluster of interacting polar residues. Unexpectedly, we find th... More

關鍵詞

E. coli,Tat protein transport,biochemistry,biophysics,membrane protein,sequence co-evolution,structural biology,twin-argi
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