The accumulation of damage caused by oxidative stress has been linked to aging and to the etiology of numerous age-related diseases. The longevity gene， sirtuin 6 (SIRT6)， promotes genome stability by facilitating DNA repair， especially under oxidative stress conditions. Here we uncover the mechanism by which SIRT6 is activated by oxidative stress to promote DNA double-strand break (DSB) repair. We?show that the stress-activated protein kinase， c-Jun N-terminal kinase (JNK)， phosphorylates SIRT6 on serine 10 in response to oxidative stress. This post-translational modification facilitates the mobilization of SIRT6 to DNA damage sites and is required for efficient recruitment of poly (ADP-ribose) polymerase 1 (PARP1) to DNA break sites and for efficient repair of DSBs. Our results demonstrate a?post-translational mechanism regulating SIRT6， and they provide the link between oxidative stress signaling and DNA repair pathways that may be critical for hormetic response and longevity assurance.